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Description

Activation of Liver AMPK with PF-06409577 corrects NAFLD and lowers cholesterol in rodent and primate preclinical models

Colocalization with automatic thresholding (ImageJ/Fiji, v1.53t, NIH) is based on a pixel-by-pixel measure of the Pearson correlation of intensities after automatic subtraction of pixels with zero correlation

The viability of the cells was spectrophotometrically measured (measuring fluorescence, excitation at 544 nm and emission at 590 nm) using an EnSpire microplate reader (Perkin Elmer)