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The evolutionary history was inferred with MEGA X (Kumar et al, 2018), sequences were aligned using the MUSCLE algorithm, and a phylogeny was generated using the maximum likelihood method with the optimised Whelan and Goldman + Freq

We used a 5-mL reaction solution in our experiments

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Emerging evidence suggests that BL can abnormally activate TRPM2 channels, leading to pathological calcium influx and microglial overactivation