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For cell colony formation assay, cells were seeded in 6-well plated at a density of 12 10 3 cells per well

Quicker graying occurs due to reduced melanin production, influenced by genetics

The construct design supports expression of the GSH1 ORF under control of the TEF1 promoter, while a counter-selectable marker is expressed under control of the GSH-regulated pGSH1

167 DUBs maintain ubiquitin system homeostasis by cleaving polyubiquitin chains or completely removing ubiquitin chains from ubiquitinated proteins and then generating and recycling free ubiquitin

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