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doi: 10.1146/annurev.arplant.56.032604.144052

RNA isolation and RNA-seq mc 2 155 cells equivalent to 30 OD 600 (e.g., 30 mL of 1 OD 600 of one culture) were harvested from each sample (three growth phases each in two biological replicates) and then quickly-frozen in liquid nitrogen

Using Grx1-roGFP2 targeted to the nucleus, we performed a 48-hour timelapse imaging to track changes in the 405/488 fluorescence ratio, which correlates with E GSH ( Figure 3a and Figure 3c )