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For the final steps of mutant construction and confirmation, recombinant colonies were selected by plating on 7H10-ADC with the appropriate antibiotic/supplement and confirmed by PCR using specific primers (Supplementary Table 2 and Fig

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Quick conversions: 1.0 mg = 6 units , 1.5 mg = 9 units , and 2.0 mg = 12 units

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11 th ed