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Overall, the findings strongly support the conclusion that chronic or acute disruptions of circadian light-dark patterns impair immune competence, particularly innate immunity, cytokine signaling, with potential effects on BC development 100,101,102

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DNA cleavage by agarose gel electrophoresis Cleavage reactions were prepared to a final volume of 20 L in 100 L Eppendorf tubes and contained 400 ng supercoiled pUC19 DNA, 1 mM Na-L-ascorbate (where indicated) and 25 mM NaCl in 80 mM HEPES buffer (pH = 7.4)

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NAC significantly attenuated ROS accumulation in knockout cells (Fig