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250 of PBS containing human HGF (1.25ng) were incubated with 3 H-Hinge the central dimerization domain of HGF, in the presence of varying concentrations of 6-AH analogs between 10 ' 3 M to 10 "7 M (half-log dilutions) for 40 min at 37C

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Therefore, establishing a hypoxia-induced endothelial cell senescence model is crucial for studying these diseases