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While direct experimental evidence comparing the responsiveness of specific -cell subpopulations to SCFAs, GLP-1, or other microbiota-derived metabolites remains limited, existing resources such as HPAP (Kaestner et al., 2019), reveal clear associations between -cell maturity, chromatin accessibility, metabolic state, and the capacity to sense exogenous signals

2.11 Western blotting CLL cells were lysed in a radio-immunoprecipitation assay buffer (Millipore-Sigma) with cOmplete mini protease inhibitor tablets (Roche) and were processed to extract the total protein

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