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The cell precipitate was resuspended in Neurobasal medium (Gibco, 21103049) supplemented with 2% B-27 (Gibco, 17504044) and 0.5 mM l-glutamine, and the cells were seeded on plates pre-coated with poly-d-lysine hydrobromide (Sigma-Aldrich, P0296)

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Multiple bands are not common in the Phi class, probably thanks to more reliable gene structures inferred by the computational analysis and, as a consequence, to a more specific primer design (Figure 1)