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Beyond benzyl alcohol distribution, the physical act of freezing can also stress the glass vial and stopper seal

The injection technique is standard subcutaneous

Dourado, D

Animals in the second group were treated with one intraperitoneal injection of polychlorinated biphenyl (Aroclor 1254) 500 mg kg -1 and were sacrificed after 7 days, while those in the third group were treated with intraperitoneal injection of 3-methylcholanthrene 20 mg -1 kg -1 day -1 for two days and were sacrificed 48 hours after treatment

Calf stretches, hamstring stretches, and gentle leg movements help maintain muscle flexibility and circulation