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Vallabhapurapu S, Karin M (2009) Regulation and function of NF-B transcription factors in the immune system

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After sonication (2 pulses of 5 s at 4 C), the cell debris was separated by centrifugation at 1000 g for 10 min, and the supernatant was ultracentrifuged at 80,000 g for 30 min at 4 C