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Ion channel activity modulation by the SARS-CoV-1-ORF3a protein also modulates viral release [39]

[ 13 C 4 ]GABA labelling of T H 17, iT reg and T H 1 cells Naive CD4 + T cells isolated from WT mice were polarized for 72 h under T H 17, iT reg or T H 1 culture conditions before being collected and re-seeded at a density of 2 10 6 cells per ml in a conditional medium (RPMI-1640) containing 0.5 mM [ 13 C 4 ]GABA, 0.1 mM Gln and the GABA A -R antagonist bicuculline (5 M)

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Spicer JD, Shewale J, Correa AM, Heymach JV, Antonoff MB , Hofstetter W, Mehran R, Roth J, Rice D, Sepesi B, Vaporciyan AA, William W, Walsh G, Swisher S