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2B), we tested whether the decrease in GCLC expression is mediated by the regulated Ire1-dependent decay (RIDD) activity of IRE1 through its RNase domain 18

Gene expression was quantified using SYBR green real-time PCR master mix (TOYOBO, Japan) and amplified under the following conditions: 2 min at 95C, 20 s at 95C, and 20 s at 58C, for 40 cycles

japonicum -infected mice, G-MDSC was found to inhibit Tfh cell proliferation, possibly through the programmed cell death protein 1 (PD-1)/programmed cell death ligand 1 (PD-L1) pathway (255)

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