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Mitophagy, a highly regulated multistep process, selectively degrades damaged or dysfunctional mitochondria

After 10 min incubation on ice, 600 l ChIP dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris-HCl/pH 8.0 and 16.7 mM NaCl, protease inhibitors) was added and genomic DNA was sheared by sonication to 3001000 bp long

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The authors attributed these effects to the elimination of ROS and inhibition of NF-B and mitogen-activated protein kinase (MAPK) signaling pathways