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Alterations in mitochondrial function in blood cells obtained from patients with sepsis presenting to an emergency department

Code 321(p)(1)

Scoring fitness and vitamin B 6 remediability Each sequencing experiment contained not only libraries derived from selective and non-selective pools, but also libraries derived from wildtype amplicons, enabling estimation of the component of each mutations observed allele frequency in the pool that was derived from PCR errors during library preparation or sequencing errors

We reasoned this route could exploit the Cys of the dipeptide to spontaneously couple with thioesters, analogous to native chemical ligation (NCL) 27,28