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Tissue Preparation Organ tissue was fixed in 10% neutral buffered formalin (pH 7.4) at room temperature for 24 h (brain, liver, kidney, lungs, heart, stomach, intestines)

One study published in the Journal of Alternative and Complementary Medicine tracked the effects of oral glutathione supplements and found no significant changes in the biomarkers of oxidative stress (20)

Equal amounts of protein lysates were loaded on Novex WedgeWell Tris-glycine or Bis-Tris gels (ThermoFisher) and blotted onto nitrocellulose or PVDF Trans-Blot Turbo membrane (0.2 m, Bio-Rad) according to standard protocols