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However, the groundbreaking discovery by Shinya Yamanaka in 2006 demonstrated that adult somatic cells can also be reprogrammed to a pluripotent state, resulting in the generation of iPSCs [88]

Data availability All data associated with this study are present in this paper or the supplementary information

Pharmacological aspects of growth hormone replacement therapy: route, frequency and timing of administration

The next day, cells were transfected with siRNA by combining 10l of 20M siRNA and 5L Dharmafect (Dharmacon, T-2001-03) in 800L OptiMEM (Gibco, 31985-062) and adding to a 6-well plate in RPMI media to a total volume of 4mL, for a final siRNA concentration of 50nM