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[36]: Since the use of the Ct method requires, among other things, the amplification efficiencies of the GST genes and the RNaseP reference genes to be approximately the same and close to 100% for singleplex reactions and multiplex reactions [36], a sample with a 1/0 genotype was established for GSTM1 and GSTT1 to create a standard curve to determine the amplification efficiencies