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This was to see how GcvA initiates transcription of the glycine biosensor by binding to the gcvBp promoter, how GcvR inhibits GcvA binding to the promoter and how glycine stops GcvR binding to GcvA

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Next, the sample was allowed to stand for 1 h at 20 C, before centrifuging at 4 C at 12,000 rpm for 15 min

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