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Sikiri et al., Dig

HBV DNA levels impact the prognosis of hepatocellular carcinoma patients with microvascular invasion

Example: 180lb person (82kg) for injury recovery at 5 mcg/kg: 82kg 5mcg = 410mcg per day With 5mg vial + 2ml water = 2,500mcg/ml concentration Draw 0.16ml per injection 12 doses per vial HOW For injury recovery (sprains, tears, tendonitis): Dose: 400-600mcg per day Frequency: Split into 2 doses (morning and evening) Duration: 4-6 weeks minimum Injection site: Near injury OR systemic (abdomen) Results timeline: 2-3 weeks to notice improvement For gut health (IBS, leaky gut, ulcers): Dose: 200-400mcg per day Frequency: Once daily, preferably morning on empty stomach Duration: 6-12 weeks Injection site: Systemic (abdomen) Results timeline: 3-6 weeks For general wellness and prevention: Dose: 150-300mcg per day Frequency: Once daily Duration: Ongoing or 4-week cycles with 2-week breaks Injection site: Systemic (abdomen) Results timeline: Subtle, cumulative benefits Body weight-based dosing: 150 lbs (68kg): Low: 204mcg (3 mcg/kg) Medium: 340mcg (5 mcg/kg) High: 476mcg (7 mcg/kg) 180 lbs (82kg): Low: 246mcg (3 mcg/kg) Medium: 410mcg (5 mcg/kg) High: 574mcg (7 mcg/kg) 200 lbs (91kg): Low: 273mcg (3 mcg/kg) Medium: 455mcg (5 mcg/kg) High: 637mcg (7 mcg/kg) Start at the low end for your goal

additionally, live-cell imaging was performed and the results analyzed according to a protocol described previously (Doanlar et al., 2020[15], 2021[16]) using the NucBlue Live Ready Probes Reagent, CM-H 2 DCFDA dye (Life Technologies, USA), and acridine orange-ethidium bromide (AO/EB) (Sigma Aldrich, USA)

Zhu H, Huang J, Chen Y, Li X, Wen J, Tian M, Ren J, Zhou L, Yang Q (2022) Resveratrol pretreatment protects neurons from oxygen-glucose deprivation/reoxygenation and ischemic injury through inhibiting ferroptosis