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Strengths of our study include the relatively large sample size, its prospective design with multiple standardised annual measurements for each patient, and a constant time between follow-up measurements

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In another study, it was reported that male mice consuming a low-protein diet produced offspring with increased level of cytosine methylation in trans -acting regulatory sequences of key genes involved in lipid and cholesterol biosynthetic pathways such as Ppar (Carone et al., In different models, it was however shown that a diet-induced stress indeed provokes changes in the DNA methylation profile of the father's sperm cells (Fullston et al., 2014) reported that a situation of diet-induced paternal prediabetes modifies the sperm methylation status of several genes such as the phosphatidylinositol (PI) 3-kinase subunits Pik3ca and Pik3r1 of the insulin pathway