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17,18,19 Although increasing the strength of stimulation through the TCR can license T cell glucose import in the absence of co-stimulation, the magnitude of glucose import in this setting is markedly diminished

The slides were incubated with primary anti-CD3 antibody, anti-CD4 antibody, and anti-CD8 antibody at 4 C overnight, which was followed by incubation with goat anti-rabbit IgG Alexa Fluor 488 at room temperature for 1 h