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To validate these findings, we quantified the mRNA levels of these genes using qRT-PCR

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Intensity of behavioral disturbances and seizure presentation assessment After paracetamol application, intensity of progressing behavioral disturbances and seizure presentation was continuously monitored, and assessed and scored (score 0 - normal, score 1 - rearing on hind legs, shaking, 2 - sleepy, tottering walk, 3 - sleepy, tottering walk and repeatedly falling down, 4 - sleepy, tottering walk and repeatedly falling down, jerking of the extremities, 5 - lying down, generalized convulsions) at particular time points depending on the regimen used, as described before

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Observation timelines vary depending on experimental design, cell type, and study conditions