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Description

The mRNA stability assays were performed supplementing medium with Actinomycin D (Sigma-Aldrich) at 5 g/mL (PC-3) or 1 g/mL (BT549) and performing RNA extraction using TRI Reagent (Sigma-Aldrich) at indicated timepoints

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Abbreviations: EPA = Eicosapentaenoic acid

Consider switching sources, adding the microneedling component if not already included, or exploring alternative approaches

Haq M, Gonzalez N, Mintz K, Jaja-Chimedza A, De Jesus CL, Lydon C, Welch A, Berry JP (2016) Teratogenicity of ochratoxin A and the degradation product, ochratoxin , in the zebrafish ( Danio rerio ) embryo model of vertebrate development