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After RNA interference (RNAi) of HrGSTm1 , the mortality and feeding duration increased, while oviposition rate, weight of eggs laid and engorged body weight decreased, although only the engorged body weight was significantly different (t-test, t (44) = 2.886, P = 0.006)

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The collected supernatant was centrifuged (160 g, 5 min), and the precipitate was resuspended in D-hanks and filtered through a 70 m cell strainer (352350, Falcon, Corning, NY, USA)

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