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RNA extraction and sequencing RNA was extracted using the Invitrogen RNAqueous Total RNA Isolation Kit, with the addition of a DNase step (Invitrogen rDNase I) to remove genomic DNA contamination

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Additionally, Zhou et al (112) examined the effect of miR-1307 on the cell cycle and chemosensitivity of OCSCs

When Conventional Pain Treatment Fails If pain persists despite medications, injections, or physical therapy, it is often a sign that: Inflammation is being driven by systemic factors Tissue damage is not healing adequately The nervous system remains stuck in a pain-sensitive state Foundational supports like nutrition, sleep, and recovery are insufficient At this point, continuing to suppress symptoms without addressing root causes can prolong suffering rather than resolve it

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