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To introduce the C-terminal Azo-tag into the coding region for the sfGFP reporter protein, two Gly codons and the amber stop codon were inserted between the C-terminal Strep -tag II and the final ochre stop codon, thus placing Pap as the last encoded residue in the reading frame, and this gene cassette was subcloned on pSB19 between the Xba I and Hin dIII restriction sites

Notably, both activation of the UPR and altered ROS production were reported in misfolded diseases like tauopathies including Alzheimers disease 12,13,14,15 , -synucleinopathies including Parkinsons disease 16 , Huntingtons disease 17,18 , and prion disease 19,20

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Glutathione plays a key role in liver detox pathways , including: Binding to heavy metals (mercury, lead, cadmium) to assist in their removal

Endocrinol Metab Clin North Am