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In contrast, the lower chambers were supplemented with RPMI 1640 or McCoys 5 A medium supplemented with 15% FBS to detect tumor cell migration and invasion ability

Hypoxia also influences iron regulation by inactivating cytoplasmic prolyl hydroxylase domain (PHD) proteins, for which iron acts as a necessary cofactor

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10.1016/0002-8223(94)92371-x [DOI] [PubMed] [Google Scholar] Hartmann A-M, Tesch D, Nothwang HG, & Bininda-Emonds ORP (2013)

Further research is needed to elucidate the underlying mechanisms and establish optimal treatment protocols