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Description

Cell suspension was plated onto poly-D,L-ornithine-coated plates with DMEM, 1ITS (Life Technologies), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.5% FBS and 0.05% gentamicin (Sigma), 10 ng/ml PDGF and 10 ng/ml FGF (Peprotech), with full medium change the next day and half medium change every other day