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Supporting the therapeutic relevance of GlyT1 blockade in ischemic models, ex vivo experiments have demonstrated that NFPS and similar inhibitors showed significant neuroprotection in OGD-induced ischemic models

Primary antibodies were diluted in TBST skimmed milk 5% buffer and the incubation was performed overnight at 4C using an orbital shaker followed by 2 h at room temperature with the secondary horseradish peroxidase (HRP)-linked antibody (Goat Anti-Mouse or Anti-Rabbit IgG Peroxidase Conjugated by Thermo Scientific)

Krubphachaya P, Juricek M, Kertbundit S (2007) Induction of RNA-mediated resistance to papaya ringspot virus type W

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