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In a previous study 45 we have assessed the quality of a number of reference genes for lung transcriptomes derived from Genevestigator V3 68 and found that DHX8 was a reliable reference gene in this context

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Immunohistochemistry and immunofluorescence Parts of each snap-frozen tissue block (including constrained and unconstrained explants) were sectioned at 5 m and 30 m thickness in the same cryostat, and post-fixed in acetone for 10 min at 20 C