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Furthermore, mitochondrial dysfunction promotes NLRP3 inflammasome assembly and caspase-1 activation, leading to the secretion of pro-inflammatory cytokines like IL-1, which are associated with the aberrant activation of CDK5 [53, 54]

This could introduce challenges for patients with lifelong anticoagulation needs, such as those with heart failure and atrial fibrillation (Yasueda et al., 2016) and could interact with antihypertensive drugs to cause an excessive drop in blood pressure, necessitating careful monitoring and dose adjustments (Zhao et al., 2022)

For example, while almost all assays rely on ApoC-II mediated activation of LPL, the source of ApoC-II can range from serum samples taken from individuals or a pool of healthy individuals ( However, it is important to note that even following our recommendations, the 3 H-triolein-based assay still requires specialized equipment and accommodations for using radioactive isotopes and remains low-throughput compared to the 96-well plate format colorimetric and fluorometric methods of detecting LPL activity

Pregnant or breastfeeding individuals (unless approved by a provider)

ApoC-II was added at a concentration of 1 M to increase LPL activity