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Western blotting Frozen cortex or hippocampal samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail

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Think of it like prepping the canvas before the artist starts painting

PloS One 7 , e46037 (2012)