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Shin H, et al

The content of GSSG was determined using GSH cleanup reagents to remove GSH from the sample

Despite the limited research available on the interplay between mitochondria and iron overload, their critical function in maintaining iron homeostasis and managing associated damage underscores their importance in the pathological processes of both congenital and acquired iron overload

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The complex was concentrated to 0.5 ml using Ultra Centrifugal Filter (ThermoFisher MWCO, 100 kDa) and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 5 M ligands, 0.00075%(w/v) LMNG, 000025% glyco-diosgenin (GDN, Anatrace) and 0.00015% (w/v) CHS to separate complex from contaminants