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After 30 min of incubation at 37 C, cells were washed three times with DPBS, and fluorescence was measured using a BD FACS Aria Fusion flow cytometer at 485/535 nm and 550/600 nm excitation/emission wavelengths

Briefly, human PAECs isolated from the main branch of the pulmonary artery of young male donors were cultured in fibronectin-coated (5 g/cm 2 ) flexible silicon chambers

Ye M, Hu C, Chen T, et al

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An alpha4 integrin-paxillin-Arf-GAP complex restricts Rac activation to the leading edge of migrating cells