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Possible antitumor mechanism of MCNs/Ln/GD/FR NPs To investigate the underlying mechanism by which MCNs/Ln/GD/FR NPs inhibit melanoma tumor growth, single-cell RNA sequencing technology was employed to analyze the compositional changes in melanoma tumor cells derived from mice in both the MCNs/Ln/GD/FR and PBS groups

L-NAME and BPC 157 regimens and dose application were adjusted according to our previous studies (reviewed Alternatively, to perceive the effect of the given medication itself (without L-NAME), rats received only BPC 157 (total dose 10 g/kg or 10 ng/kg as retrobulbar application in each eye, 1 g/0.1 ml saline/each eye, or 1 ng/0.1 ml saline/each eye) or only saline via retrobulbar application in each eye (0.1 ml/each eye) (as an additional control sham group) at the corresponding time point

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Exosomes were observed by TEM (FEI Tecnai G2 Spirit BioTWIN) after drying at room temperature

SPSS v.28 (IBM Corporation) and Prism v.9 (GraphPad Software) were used for the statistical analysis