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After culture for 40 h, cells were collected and lysed in 8 ml of hypotonic buffer (10 mM HEPES pH 8.0, 10 mM KOAc, 1.5 mM Mg(OAc) 2 , 2% (v/v) glycerol, 5 mM NDSB-256 and 0.5 mM TCEP, with 1 tablet of cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001) per 10 ml of buffer) by a Dounce homogenizer

doi: 10.1101/cshperspect.a019497 206 LuXDengYYuDCaoHWangLLiuLet al

10.1155/2020/7627934 6 BaeY

Eosinophils and functional subsets based on transcriptomic profiling Our transcriptomic profiling revealed a previously uncharacterized inflammatory transcriptional trajectory in skin-infiltrating eosinophils

Guiyun Jin, [email protected] These authors have contributed equally to this work and share first authorship Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers