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From this analysis it was apparent the plasmids contained one of two different size inserts, with the smaller of the two roughly corresponding to the predicted insert size of the correctly spliced transcript

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In analytical gel filtration on a Superose 6 column, both CBS FL and CBS FL-CHis proteins eluted as a broad peak, suggestive of different oligomeric states with molecular weights larger than tetramers possibly up to the range of 1837 mDa and potentially even larger (Supplementary Fig