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Other sirtuins, including SIRT2, SIRT4, SIRT5 and SIRT6, have a K m for NAD + below the physiological range, implying that NAD + might not necessarily be the rate-limiting of their activity

Sengling Cebin Coppa et al

The shoot and root portions of plants were cleansed with sterile water, excess water was eliminated, and they were quickly frozen using liquid nitrogen