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Infarct size was assessed after 30 min of ischaemia followed by 24 h of reperfusion using Evans blue staining (1%) for delineation of the ischaemic area at risk (AAR) from the non-ischaemic zone (remote area) and 1% 2,3,5-triphenyl tetrazolium chloride (TTC) staining for demarcation of the viable and non-viable myocardium within the AAR, as described 19

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J Exp Bot 72(3):830847 Schwarzlnder M, Dick TP, Meyer AJ et al (2016) Dissecting redox biology using fluorescent protein sensors