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Signal Transduct Target Ther 2020;5:298

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Type II: more than 50% of plasma membrane was labeled with Annexin-V-EGFP

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Membranes were excised and incubated in phenol red-free Williams Medium E medium (PAN-Biotech) containing 100 M monochlorobimane (mBCI) (Sigma-Aldrich), 5 M CellROX Deep Red Reagent (Thermo Fisher Scientific) and 200 nm MitoTracker Red CMXRos (Thermo Fisher Scientific) for 30 min at 37 C and 5% CO 2