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Following this, nuclei were stained with hematoxylin for 510 min, differentiated in acid ethanol, and then cytoplasm was counterstained with eosin for 13 min

Critical knowledge gaps persist in our understanding of AT dysfunction, particularly regarding depot-specific molecular heterogeneity, temporal dynamics of dysfunction progression, and sex-specific differences in adipose pathophysiology

The homogenates were then sonicated on ice and subjected to centrifugation at 150,000 g for 30 min at 4 C, followed by purification with Glutathione Sepharose 4 Fast Flow Gel (pre-balanced with GST binding buffer) at 4 C