glutathione s transferase and prostate cancer S-transferase π: a potential role in antitumor therapy | DDDT Glutathione S-Transferases in Cancer

Virology 313 , 126135 (2003)

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Ontogeny of -and -opiate receptor control of the hypothalamo-pituitary-adrenal axis in rats

The resin was washed 3 times with lysis buffer and heated at 65 o C with 2 urea sample buffer (150 mM Tris pH 6.8, 6 M urea, 6% SDS, 40% glycerol, 100 mM DTT, 0.1% Bromophenol blue) at 65C for 10 minutes to elute bound proteins