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The PCR reaction conditions consisted of 95C for 3 min (1 cycle), 95C for 30 s and 50C for 30 s as well as 72C for 30 s (25 cycles), and a final extension at 72C for 10 min in the presence of Fast Hifidelity Polymerase and Phusion High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs Co., Ltd., Beijing, China)

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This review compiles data from global epidemiological studies and clinical practices, exploring the physiological processes and the genetic foundations of urate transporters in depth

The child was given 10 mL, or about half of the bottle, with the intent of giving him 40 mg

In Biotechnology Advances 25 (2), 207210