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When we assessed the outcome of cryopreservation in tissues frozen with various combinations of two different types of penetrating cryoprotectants, the best results were observed in prepubertal testicular tissue frozen in a medium containing 1% glycerol and 4% DMSO, which was comparable to 5% DMSO alone group

Subsequently, 12.12 mg of triethylamine, serving as the base catalyst, were added to the solution, and the mixture was stirred at room temperature for 10 min

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