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Ferroptosis is also associated with excess Fe3+ in the intracellular medium

Hypoxia also led to the decreased conversion of pyruvate to acetyl-CoA, which was restored by the knockdown of ECHS1 (Supplementary Fig

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To exclude non-specific hits, we further validated these hits in TNF/cycloheximide (CHX)-induced HepG2 liver cell pyroptosis model, which was mediated by caspase-3-GSDME pathway 20