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After treatment, the cells were stained with 5 M MitoSOX Red in HBSS/Ca/Mg solution, incubated for 10 min at 37 C and washed with prewarmed HBSS/Ca/Mg solution three times

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The samples were enriched by adding 1% volume to 50 ml of sterile de Man Rogosa Sharpe (MRS) broth and incubated at 37C for 48 h

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