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However, the biosynthesis of -glutamyl tripeptide is commonly reported through the ligation of glutamate with various amino acids via gamma-glutamylcysteine synthetase (GCS) and glutathione synthetase (GSS) 28 (Fig

Indeed, the determination of the perinuclear mitochondrial CMFDA staining after co-localization with mitotracker red (as described previously [24]) (figure 4C) in 3T3 fibroblasts at 24 hours of culture show that BSO treated cells maintained a high mitochondrial CMFDA staining but cytosolic fluorescence was indeed decreased by BSO

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To dock ligands into a receptor binding site, a high-throughput site-featured algorithm was used with the docking software LibDock(hotspot-directed- high throughput docking), which is provided by DS with default parameters except for docking performance (Fast search), conformation method (Fast), and minimization algorithm (do not minimize)

11.1, 6-MP is inactivated by two pathways