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10.3892/etm.2016.3079 33 DingH.WeiJ.ZhaoY.LiuY.LiuL.ChengL

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"Current Trends in the Management of Gastroesophageal Reflux Disease"

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Cells were lysed in radioimmunoprecipitation assay buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate and 0.1% SDS) and protein content was measured using a standard BCA kit (Beyotime, P0009)