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Hydrogen-bond distance, and dihedral-angle restraints from the TALOS+ (v3.70F1) prediction were added at a later stage of structure calculations for residues with characteristic NOE patterns

Ljubimov, L.L

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Separation and Quantification of SAM and SAH by LC-MS/MS The concentrations of SAM, SAH, and Crea from solutions prepared as described in the methods (Figure 2a) could be determined simultaneously by reverse-phased LC using isocratic solvent conditions and MS as a detection system